Sequential fed batch extractive fermentation for enhanced bioethanol production using recycled Spathaspora passalidarum and mixed sugar composition

The simultaneous ethanol production and removal during sequential cell recycle fed batch fermentation provides a complementary route to produce this biofuel from sugar mixtures, which may greatly improve yields productivity lignocellulosic hydrolysates. Spathaspora passalidarum is wild-type strain able naturally convert glucose, fructose, xylose arabinose into ethanol. Therefore, the present work has focused on 2G bioethanol by S. aiming at consumption of all sugars released after pre-treatment enzymatic hydrolysis sugarcane bagasse in single step. strategy with recycle, fed-batch mode situ was performed hemicellulosic hydrolysate medium supplemented molasses. This gave improved performance enabled co-fermention under microaerobic conditions. maximum yield 0.482 g.g?1 9.5 g·L-1·h?1, respectively, showing process efficiency 94.3%. selective enables operation bioreactor low levels (20–30 g·L-1), even high concentration inputs, accelerating avoiding inhibitory effects yeast metabolism. Applying strategy, increase its robustness, as shown 10-fold productivity, it also tolerate acetic acid (4.5 g·L-1) long-term fermentations. These results demonstrate that bioprocess strong potential rich mixed hydrolysates